Dear Peter, this question is not dumb. If complete antibodies were used, one should have amplified the other (and vice versa). That's what people usually do to amplify signals!
Apparent differences in the stained structures (I assume some sort of detection by photography, video or confocal scanning microscopy), simply may arise from different sensitivities in the optical channels used. One possibility I can imagine where such an experiment gives reliable results is, when the secondaries are directed against the other species' Fc, but missing their own Fc (means they are incomplete antibodies), which might be quite unusual/uncommon. HTH Wo _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
