Cytoclear is a product we have successfully used over a few years which can effectively dissolves and remove cytoplasm around mamalian & human cells. In particular, according to the cimpany's literature: "it dissolves the lipid and glycoprotein structure of which cytoplasm is mainly composed. Clumped metaphases that would normally be impossible to analyse can now be restored. Cytoclear is a useful tool in FISH hybridizations as it enables the probe to reach the target DNA by removing barrier cytoplasm surrounding the cell."
The company link to Cytoclear is below, i highly recommend it and worth a go http://www.genialgenetics.com/products/reagents/cytoclear-cytoplasm-remover.html _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
