Since you are studying platelet adhesion, do you need red blood cels around. YOu could remove them by sedimenting them out and then use the plasma for your flow studies. jay
________________________________________ From: [email protected] [[email protected]] On Behalf Of Joshua Silverstein [[email protected]] Sent: Tuesday, August 24, 2010 6:52 PM To: [email protected] Subject: Re: Blood is settling Hi all - I sent this a while ago and never really found a response that seemed like a viable option. Could anyone suggest some other perturbation that may inhibit red blood cell sedimentation? I'd prefer to not add any solutes (unless they are entirely benign) to the blood like others suggested. Thanks Josh On Thu, Jul 29, 2010 at 11:09 AM, Joshua Silverstein < [email protected]> wrote: > Hi again - > > I'm running blood over some surfaces under flow with a syringe pump. The > problem is that the blood seems to settle extremely quickly when it is on > it's side in the pump. A colleague suggested putting a stir bar inside the > syringe but I am worried about activating the platelets and since the > primary objective of this experiment is to assess platelet adhesion, I don't > know if that's a good idea. Any other suggestions or at least a maximum > speed so that I do not skew the results? > > Thanks > Josh Silverstein > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
