That is ingenious. I was fretting that the gene or peptide sequence for SpeI is apparently not known or available online, and access to that particular bacterial strain would be very costly. If I can transform from sacI enzyme and get the plasmid for sequencing, it will be a joyous occasion!
--- Twitter: @onetruecathal Sent from my beloved Android phone. On 27 Jul 2010 17:46, "DK" <[email protected]> wrote: In article <[email protected]>, jh < [email protected]> wrote: >Dear al... Have really highly competent cells and possibly try several Taq suppliers: "transform" cells with the protein prep. Virtually every recombinant protein prep is contaminated at a very low level with the recombinant plasmid. Since it is not known what resistance marker was used, plate on amp, kan and cam plates. I have used this trick to get several genes. DK _______________________________________________ Methods mailing list [email protected] http://... _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
