Hi Methodologists - I want to do a Live/Dead Assay on some HUVECs and the protocol calls for using saponin to kill control cells. I noticed it is a surfactant, can I use something like Tween-20 or Triton-X 100 instead that I have around the lab? Also what concentration would you use (I usually do 1% Triton to permeate the cells)?
Thanks all, Josh _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
