In article <[email protected]>, [email protected] says... > > Hi all, > I'm trying to set up some new in situ in the lab using antibodies rather than > radiolabelling my oligos, I have been told this is not as sensitive or as > good...does anyone have any thoughts? I'm rather fed up of developing in > situ films over a week, it seems a little old fashioned!
Yes, try chemiluminescence. The detection reagent (oligo, antibody, lectin...) is labelled with a meta-stable compound which decomposes under defined conditions (rapid switch between acidic and basic) within 1 s, emitting light that is counted. You need an injecting luminometer, e.g. from Berthold. You have about 1 label molecule per macromolecule, similar to iodination. However, all those labels decompose in a second, rather than over weeks. That results in an increased sensitivity and convenience. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
