Hi everyone,
I recently ran a 2-8% gradient polyacrylamide gel. The 1kb Plus DNA
ladder had multiple bands. Does anyone know what might have caused
this and how to prevent it? I suspect that I might be caused by the
freeze thaw cycle. Also, some of my bands of my PCR samples were
slanted using a pre-cast gel. How do you prevent that? (I washed out
the wells thoroughly before use) Thanks.
-Graduate student
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