Hello all,
I have to isolate G-PCR (G-protein coupled receptor) from ovarian cancer cell
line. I am looking for a protocol (buffer,etc) that not use
extraction/isolation kit (I could mix the buffer by my self) and not change its
native structure. There is a problem that my ultracentrifuge only can spin up
to 15,000 g. I hope anybody could help me. Thank you very much.
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