On Feb 2, 2010, at 6:16 AM, Nick Theodorakis wrote:
On Feb 2, 5:26 am, WS <[email protected]> wrote:
Dear Experts,
is there any commercial (and preferably cheaper) alternative to PD-10
desalting columns?
(They are filled with Sephadex G-25, sample volume is up to 2.5ml,
elution volume is 3.5ml).
Buy the empty disposable columns and fill them yourself.
Alternatively, if you can work with low (100 ul) volumes, you can pour
them in 1cc syringes and do spin-column desalting.
One doesn't even need to limit oneself to a small volume. I've run
spin columns with 3 and 5 mL syringe barrels. I've found I can easily
apply up to 1/3 of matrix volume, get good recovery and good
desalting. Details of my column procedure can be found in Crop
Science 46:662-670 (2006).
Mike
---
Michael L. Sullivan
Plant Research Molecular Geneticist
US Dairy Forage Research Center
ARS-USDA
1925 Linden Drive West
Madison, WI 53706
(608) 890-0046 (Phone)
(608) 890-0076 (FAX)
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