Welcome to the world of IHC. They only like nice in publications. You got to
keep trying different antibodies before you can get one that works. Every
company claims that their antibodies work, but take that with a pinch of salt.
Usually, they don't. Also check out literature and contact people how did it
well. If you are the first one, tough luck. You just got to keep trying out
different antibodies. Each time, try out different concentrations, dilutions
etc. Everything matters in IHC. If you can outline the protocol that you are
using, that will be a good start.
Jay
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of Scott Brown
Sent: Sunday, January 10, 2010 5:34 PM
To: [email protected]
Subject: IHC with Nalm-6
Hi all,
I have been trying to do some IHC and IF using Nalm-6 cells, on every occasion
my controls look identical to the one I have used antibody on.
Does anyone know if Nalm-6 cells express surface immunoglobins that my
secondary may be binding to?
Regards
Scott
Scott Brown
PhD Candidate
Molecular Carcinogenesis Program
Children's Cancer Institute of Australia for Medical Research Lowy Cancer
Research Centre, UNSW High Street (PO Box 81) RANDWICK NSW 2031 AUSTRALIA
Phone: +61 2 9382 1829
Fax: +61 2 9382 1850
Email: [email protected]
Web: www.ccia.org.au <http://www.ccia.org.au/> Children's Cancer Institute
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