Dear all, i'm unable to transform my desired gene in the yeast shuttle vector pRS416. after ligation, when i transform this mix in DH5a cells, there are no colonies observed. moreover the cell mass can be observed in the form of a dirty smear over the plate. i have changed the vector stock, the competent cell stock bt it just doesnt transform. the vector control plate also shows no colonies, bt as soon as i ligate the insert to this vector, the cell mass is observed. wat could be the reason for this??
Mary ([email protected]) research scholar IMTECH _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
