I need the stain to be non fluorecent because I dont have acess to a fluorecent microscop. I know Haematoxylin, but as I anderstand, using this stain must involve fixation. I am working with living cells and I am trying to find a way to stain them without fixation, unless there is no choice. Is there a way that you know?
2009/12/28 Nick Theodorakis <[email protected]> > On Dec 27, 9:51 am, Sébastien Vigneau <[email protected]> > wrote: > > Haematoxylin? > > I was thinking of that, too. > > Some clarification from the OP would help, too. Is it that you don't > have access to a fluorescent scope, or that you don't want the stain > to be fluorescent? Because if it is the latter, I believe that > hematoxylin is autofluorescent; or at least, some component of an H&E > stained slide is autofluorescent. > > What is the nature of your sample? Live, fixed or frozen? Cells or > tissues? How are you visualizing it now? > > Nick > > -- > Nick Theodorakis > [email protected] > contact form: > http://theodorakis.net/contact.html > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
