Hydrolysis by autoclaving SDS I haven't heard of, but it is a common occurrence that SDS precipitates following autoclaving. I would look at the water you are using. The K-salt is insoluble and autoclave environment may be introducing some potassium salt. Is it stll insoluble after you let it sit at room temp?
> It could be hydrolysis of SDS occurring during autoclaving, producing > sulfuric acid and decanol. If you can use a buffer, I highly suggest > that.. > The reaction that occurs is further catalyzed by the product, thus it only > gets faster and faster. Hope this helps. > > Josh > > On Fri, Dec 18, 2009 at 12:06 PM, Duncan Clark > <[email protected]>wrote: > >> Hi Folks, >> >> Anyone any idea why if one autoclaves 10% (w/v) SDS prepared in MilliQ >> water for 25mins instead of 15mins, it will no longer go back into >> solution >> even when diluted 100fold into warm water? Biorad is the source a. If >> you >> know how to get it back into solution, please tell. >> >> I've never come across this before. Most odd. >> >> Duncan >> -- >> I love deadlines. I especially like the whooshing noise they make as >> they go flying by. >> >> Duncan Clark >> GeneSys Ltd. >> _______________________________________________ >> Methods mailing list >> [email protected] >> http://www.bio.net/biomail/listinfo/methods >> > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > -- Hiranya S. Roychowdhury, Ph.D. Asst. Professor, Health & Public Services Dona Ana Community College New Mexico State University Las Cruces, NM 88003 _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
