methods

Bionet discussion lists will be turned off this week

Wed, 03 Apr 2019 17:44:01 GMT

2019/04/03 -- Don Gilbert

This Bionet discussion list will be turned off in April

Sat, 16 Mar 2019 18:38:42 GMT

2019/03/16 -- Don Gilbert

Bionet at 30 years of open science communication

Sun, 17 Feb 2019 18:16:56 GMT

2019/02/17 -- Don Gilbert

Stable Transfection of NIH-3T3 and 3T3-L1

Thu, 14 Feb 2019 02:31:39 GMT

2019/02/14 -- sushilapthk60

Re: Imidazole and Amicon concentrators

Thu, 27 Dec 2018 12:20:32 GMT

2018/12/27 -- Wolfgang Schechinger

Imidazole and Amicon concentrators

Thu, 27 Dec 2018 01:30:24 GMT

2018/12/27 -- m . golgoljaam

Re: Request for dam–/dcm– E. coli

Fri, 07 Dec 2018 19:35:29 GMT

2018/12/07 -- Björn Johansson

Request for dam–/dcm– E. coli

Thu, 06 Dec 2018 20:03:16 GMT

2018/12/06 -- Bjorn

Re: EGTA

Thu, 11 Oct 2018 12:31:06 GMT

2018/10/11 -- WS

Re: EGTA

Tue, 09 Oct 2018 18:18:57 GMT

2018/10/09 -- Hiranya Roychowdhury

Re: EGTA

Mon, 08 Oct 2018 22:57:26 GMT

2018/10/08 -- kkerns . mobile

Re: How to prepare 1M H2O2 using 30% hydrogen peroxide?

Thu, 27 Sep 2018 17:09:37 GMT

2018/09/27 -- thekingmbembe

Trouble with PCR

Wed, 01 Aug 2018 19:49:08 GMT

2018/08/01 -- saadlee shehreen

Stock solutions of spermine/spermidine?

Sun, 06 May 2018 19:44:26 GMT

2018/05/06 -- rajkumarm574

short oligonucleotide probe Tm and specificity analysis

Thu, 05 Apr 2018 19:56:41 GMT

2018/04/05 -- neda karami

protein concentration at 280 nm

Thu, 22 Mar 2018 12:11:01 GMT

2018/03/22 -- Sudheer Sangeetham

Re: Drop Dialysis of Ligations

Thu, 15 Mar 2018 01:10:12 GMT

2018/03/15 -- shyamals86

Potassium Phosphate Buffer

Wed, 07 Feb 2018 01:48:00 GMT

2018/02/07 -- cvthakor

Re: DNA binding proteins

Mon, 27 Nov 2017 20:56:42 GMT

2017/11/27 -- heathbowers

Re: Thioester bonds

Sun, 26 Nov 2017 19:42:07 GMT

2017/11/26 -- travisschwochert

Re: gc/ms for sphingosine

Wed, 22 Nov 2017 18:32:31 GMT

2017/11/22 -- Wolfgang Schechinger

gc/ms for sphingosine

Tue, 21 Nov 2017 21:09:46 GMT

2017/11/21 -- Ed Siefker

Re: Fwd: Slimy recombinant e.coli pellet

Tue, 21 Nov 2017 14:15:43 GMT

2017/11/21 -- Megha Goyal

Need help on bioinformatics-sequence retrieval

Fri, 17 Nov 2017 17:47:11 GMT

2017/11/17 -- Sudheendra Rao N R

Re: Slimy recombinant e.coli pellet

Fri, 17 Nov 2017 00:56:49 GMT

2017/11/17 -- P J

Fwd: Slimy recombinant e.coli pellet

Thu, 16 Nov 2017 19:59:37 GMT

2017/11/16 -- Megha Goyal

Re: pGreen Lantern sequence file

Mon, 13 Nov 2017 16:44:15 GMT

2017/11/13 -- Bjorn

Re: Slimy recombinant E. coli pellet

Fri, 10 Nov 2017 17:47:21 GMT

2017/11/10 -- MacGregor, Barbara

Re: Gel Solubilization Buffer Composition

Fri, 10 Nov 2017 17:47:16 GMT

2017/11/10 -- Hiranya Roychowdhury

Re: Slimy recombinant e.coli pellet

Fri, 10 Nov 2017 17:46:31 GMT

2017/11/10 -- P J

Re: Methods Digest, Vol 137, Issue 1

Fri, 10 Nov 2017 17:46:16 GMT

2017/11/10 -- Virash Gupta

Re: Slimy recombinant e.coli pellet

Fri, 10 Nov 2017 17:46:11 GMT

2017/11/10 -- Megha Goyal

Vacuolar dye MDY-64: Chemistry? Mode of action?

Fri, 10 Nov 2017 17:45:39 GMT

2017/11/10 -- MacGregor, Barbara

Re: Slimy recombinant e.coli pellet

Thu, 09 Nov 2017 21:34:08 GMT

2017/11/09 -- Wolfgang Schechinger

Slimy recombinant e.coli pellet

Thu, 09 Nov 2017 13:17:09 GMT

2017/11/09 -- Megha Goyal

RE: Stripped and recharged Ni-NTA beads but still getting impurities only with Ni-NTA beads

Sat, 05 Aug 2017 20:11:01 GMT

2017/08/05 -- Phelan, Paul J.

Stripped and recharged Ni-NTA beads but still getting impurities only with Ni-NTA beads

Fri, 04 Aug 2017 18:17:12 GMT

2017/08/04 -- Sudheer Sangeetham

Re: Phosphate-Buffered Saline precipitate when adding NaOH

Thu, 13 Jul 2017 16:01:16 GMT

2017/07/13 -- Dmitry Bochkariov

Phosphate-Buffered Saline precipitate when adding NaOH

Wed, 12 Jul 2017 17:15:42 GMT

2017/07/12 -- najib abdellaoui

Need a book "Liposomes: A Practical Approach"

Thu, 15 Jun 2017 21:51:27 GMT

2017/06/15 -- Atul Tripathi

Re: what is the milliQ water pH?

Wed, 07 Jun 2017 20:07:42 GMT

2017/06/07 -- Dmitry Bochkariov

Re: what is the milliQ water pH?

Wed, 07 Jun 2017 16:14:57 GMT

2017/06/07 -- Wolfgang Schechinger

what is the milliQ water pH?

Tue, 06 Jun 2017 19:20:36 GMT

2017/06/06 -- Sudheer Sangeetham

pH of urea increasing with increasing concentration

Thu, 01 Jun 2017 19:16:53 GMT

2017/06/01 -- Sudheer Sangeetham

request for pFG and pFL vector map and DNA sequence.

Tue, 23 May 2017 18:46:56 GMT

2017/05/23 -- Ramachandran.B.

Re: protein aggregation

Sat, 20 May 2017 21:21:56 GMT

2017/05/20 -- Wolfgang Schechinger

protein aggregation

Fri, 19 May 2017 18:21:08 GMT

2017/05/19 -- TieQiao Wu

Biocuration - Portal For Sharing Life Science Information

Wed, 17 May 2017 15:37:14 GMT

2017/05/17 -- Mohammed Iftekhar

Re: DNA binding proteins

Thu, 02 Mar 2017 17:55:15 GMT

2017/03/02 -- Bjorn

DNA binding proteins

Wed, 01 Mar 2017 16:00:07 GMT

2017/03/01 -- Renato De Souza Pinto Lemgruber

Re: UV 365 nm, Photo crosslinking on ice?

Thu, 08 Dec 2016 17:06:05 GMT

2016/12/08 -- Dmitry Bochkariov

Re: UV 365 nm, Photo crosslinking on ice?

Fri, 02 Dec 2016 02:07:03 GMT

2016/12/02 -- Hiranya Roychowdhury

UV 365 nm, Photo crosslinking on ice?

Thu, 01 Dec 2016 19:04:15 GMT

2016/12/01 -- Sudheer Sangeetham

Re: pGreen Lantern sequence file

Thu, 01 Dec 2016 19:03:22 GMT

2016/12/01 -- Bjorn

Re: pGreen Lantern assembly by Bjorn

Thu, 24 Nov 2016 17:50:12 GMT

2016/11/24 -- Bjorn

pGreen Lantern assembly by Bjorn

Wed, 23 Nov 2016 19:16:58 GMT

2016/11/23 -- MacGregor, Barbara

pGreenLantern-1 sequence

Tue, 22 Nov 2016 17:47:00 GMT

2016/11/22 -- Bjorn

pGreen Lantern-1 sequence assembly

Tue, 22 Nov 2016 03:39:07 GMT

2016/11/22 -- Bjorn

pGreen Lantern, possibly helpful

Sat, 19 Nov 2016 20:24:53 GMT

2016/11/19 -- MacGregor, Barbara

Re: pGreen Lantern sequence file

Fri, 18 Nov 2016 03:57:03 GMT

2016/11/18 -- Wolfgang Schechinger

Re: pGreen Lantern sequence file

Thu, 17 Nov 2016 20:25:51 GMT

2016/11/17 -- arregui . leticia

Simple PCR to linearize plasmid not working

Thu, 17 Nov 2016 02:53:04 GMT

2016/11/17 -- Phelan, Paul J.

Re: gRNA design and off target selection

Thu, 06 Oct 2016 19:04:08 GMT

2016/10/06 -- Vladimir Gainullin

gRNA design and off target selection

Wed, 05 Oct 2016 18:17:54 GMT

2016/10/05 -- zhyjiang2006

C1R stable transfection

Wed, 28 Sep 2016 16:16:29 GMT

2016/09/28 -- Cano, Enrique

help: plasmid extraction with unknown contamination

Wed, 21 Sep 2016 18:16:59 GMT

2016/09/21 -- Bing Dai

Re: 293T cells derivative which needs medium with supplement and would not grow otherwise

Sun, 31 Jul 2016 17:39:11 GMT

2016/07/31 -- Christian Praetorius

Re: Air bubbles in my agar plates

Tue, 19 Jul 2016 18:30:12 GMT

2016/07/19 -- mincheol . park88

Phusion 5xbuffer

Tue, 14 Jun 2016 18:03:18 GMT

2016/06/14 -- m.kroiher

Re: skim milk agar

Fri, 27 May 2016 20:41:57 GMT

2016/05/27 -- askintekin22

skim milk agar

Sun, 15 May 2016 17:34:07 GMT

2016/05/15 -- Sue Katz

Skim milk agar hints

Fri, 13 May 2016 20:30:43 GMT

2016/05/13 -- MacGregor, Barbara

Query about skim milk agar

Thu, 12 May 2016 21:43:12 GMT

2016/05/12 -- sairahbano47

293T cells derivative which needs medium with supplement and would not grow otherwise

Tue, 10 May 2016 18:05:12 GMT

2016/05/10 -- Alfredo Pagliuca

Re: ceq 8000 lpa

Thu, 10 Mar 2016 19:28:40 GMT

2016/03/10 -- chovek69

ceq 8000 lpa

Wed, 09 Mar 2016 17:37:32 GMT

2016/03/09 -- james jurgenson

pcr

Wed, 09 Mar 2016 17:36:27 GMT

2016/03/09 -- Manali Chakraborty

Request for software

Fri, 04 Mar 2016 20:55:31 GMT

2016/03/04 -- OLAIDE YEKEEN

mitotic spreads preparation

Sun, 28 Feb 2016 18:47:22 GMT

2016/02/28 -- pasquale pensieri

transient expression trouble

Tue, 23 Feb 2016 17:44:00 GMT

2016/02/23 -- pasquale pensieri

Re: pGreen Lantern sequence file

Fri, 12 Feb 2016 03:30:06 GMT

2016/02/12 -- jjduke11

Re: Cytofluor II (was: "Fluoroscan II software")

Tue, 19 Jan 2016 18:17:37 GMT

2016/01/19 -- kstumpo

Re: the absorbance value 1.05 at 260/280 of protein solution

Thu, 29 Oct 2015 17:03:24 GMT

2015/10/29 -- Sudheer Sangeetham

Re: the absorbance value 1.05 at 260/280 of protein solution

Wed, 28 Oct 2015 20:36:00 GMT

2015/10/28 -- Pow Joshi

RE: the absorbance value 1.05 at 260/280 of protein solution

Wed, 28 Oct 2015 20:12:12 GMT

2015/10/28 -- Nair, Jayakumar

RE: the absorbance value 1.05 at 260/280 of protein solution

Wed, 28 Oct 2015 20:11:43 GMT

2015/10/28 -- Phelan, Paul J.

the absorbance value 1.05 at 260/280 of protein solution

Wed, 28 Oct 2015 17:25:04 GMT

2015/10/28 -- Sudheer Sangeetham

RE: different molecular weights proteins 23 and 66 kDa?

Sun, 25 Oct 2015 21:32:55 GMT

2015/10/25 -- Jayakumar Nair

RE: different molecular weights proteins 23 and 66 kDa?

Fri, 23 Oct 2015 18:08:42 GMT

2015/10/23 -- Nair, Jayakumar

different molecular weights proteins 23 and 66 kDa?

Thu, 22 Oct 2015 22:01:26 GMT

2015/10/22 -- Sudheer Sangeetham

Re: Dilute nitric acid for cleaning glassware and lab tools

Wed, 21 Oct 2015 17:03:15 GMT

2015/10/21 -- Dr S.A.D.P. Senadheera

Dilute nitric acid for cleaning glassware and lab tools

Mon, 19 Oct 2015 18:25:04 GMT

2015/10/19 -- John Dudgeon

Can I estimate the ' Fluorescent proteins' concentration at 280 nm ?

Wed, 30 Sep 2015 19:09:57 GMT

2015/09/30 -- Sudheer Sangeetham

RE: Na2Po2 buffer?

Fri, 18 Sep 2015 21:51:26 GMT

2015/09/18 -- Hans-Georg Koenig

Na2Po2 buffer?

Fri, 18 Sep 2015 16:52:01 GMT

2015/09/18 -- Sudheer Sangeetham

Re: Cytofluor II (was: "Fluoroscan II software")

Thu, 17 Sep 2015 19:33:15 GMT

2015/09/17 -- gettl008

Poly-deoxyribose phosphate?

Mon, 31 Aug 2015 21:35:02 GMT

2015/08/31 -- Phelan, Paul J.

Sequncing the rest of the Gene

Tue, 04 Aug 2015 16:03:55 GMT

2015/08/04 -- M Aldeeb

Re: Miscelle size of detrgent mix

Tue, 07 Jul 2015 16:13:03 GMT

2015/07/07 -- Dr Engelbert Buxbaum

Miscelle size of detrgent mix

Sun, 05 Jul 2015 17:04:35 GMT

2015/07/05 -- Imran khan